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Related Concept Videos

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Tissue Renewal without Stem Cells

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After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
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Related Experiment Video

Updated: May 6, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

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[Human stem cells from apical papilla can regenerate dentin-pulp complex].

Huacui Xiong1, Ke Chen, Yibin Huang

  • 1Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|October 23, 2013
PubMed
Summary

Human stem cells from apical papilla (SCAP) show promise for regenerating the dentin-pulp complex. When combined with HA/TCP, SCAP successfully regenerated pulp-dentin-like tissue in vivo.

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Regenerative Medicine

Context:

  • The dentin-pulp complex is crucial for tooth vitality.
  • Current treatments for pulp damage are limited.
  • Tissue engineering offers a novel approach for complex tissue regeneration.

Purpose:

  • To investigate the potential of stem cells from apical papilla (SCAP) for dentin-pulp complex regeneration.
  • To evaluate the in vitro and in vivo differentiation capacity of SCAP.
  • To assess the efficacy of SCAP in combination with a hydroxyapatite/tricalcium phosphate (HA/TCP) scaffold.

Summary:

  • SCAP isolated from human third molars exhibited mineralization potential and expressed osteo/odontogenic markers in vitro.
  • SCAP cultured with HA/TCP scaffolds successfully regenerated pulp-dentin complex-like tissue in nude mice.
  • The regenerated tissue showed positive staining for dentin sialoprotein (DSP), indicating dentin formation.

Impact:

  • SCAP represent a promising cell source for dental tissue engineering.
  • This study provides a foundation for developing new regenerative therapies for dental injuries.
  • Successful regeneration of the dentin-pulp complex could improve long-term tooth preservation.