Ozonated oils as functional dermatological matrices: effects on the wound healing process using SKH1 mice

Area of Science:

• Biomedical Engineering
• Dermatology
• Pharmacology

Background:

• Wound healing is a complex biological process critical for restoring tissue integrity.
• Chronic wounds, often seen in conditions like diabetes, present significant clinical challenges.
• Ozonated vegetable oils are emerging as potential therapeutic agents due to their antimicrobial properties.

Purpose of the Study:

• To compare the efficacy of different ozonated vegetable oils (olive, sesame, linseed) on wound healing rates.
• To investigate the impact of ozonated oils on histological parameters and key protein expression.
• To determine the role of oil composition, beyond ozonation level, in wound repair.

Main Methods:

• SKH1 mice were used to assess wound healing rates following treatment with different ozonated oils.
• Histological analysis was performed to evaluate tissue repair.
• Levels of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were measured.

Main Results:

• Ozonated sesame oil significantly accelerated wound closure within the first 7 days compared to other oils.
• This enhanced healing correlated with increased VEGF and PCNA levels.
• Increased NFκB nuclear translocation and 4-HNE formation were observed with ozonated sesame oil treatment.

Conclusions:

• Ozonated sesame oil demonstrates superior efficacy in promoting wound healing.
• Both the degree of ozonation and the intrinsic composition of the oil influence therapeutic outcomes.
• These findings suggest a promising role for specifically formulated ozonated oils in wound management.