Proteomics boosts translational and clinical microbiology
F Del Chierico1, A Petrucca2, P Vernocchi3
1Unit of Parasitology, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4, 00165 Rome, Italy; Unit of Metagenomics, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4, 00165 Rome, Italy.
Journal of Proteomics
|October 23, 2013
Summary
Proteomics offers advanced tools for infectious disease management and understanding microbial systems. This review covers proteomic protocols for pathogen characterization and diagnostics, including MALDI-TOF MS applications.
Area of Science:
- Microbiology
- Proteomics
- Infectious Diseases
Background:
- Proteomics is revolutionizing infectious disease management and the study of microbial systems.
- Understanding pathogen-host interactions is crucial for diagnostics and treatment.
- Advanced proteomic applications are emerging for pathogen profiling and resistance detection.
Purpose of the Study:
- To review the state-of-the-art in proteomic protocols for translational and diagnostic microbiology.
- To highlight the application of proteomics in characterizing pathogens and microbiota.
- To discuss MALDI-TOF MS applications in advanced diagnostics for emerging infections and fastidious bacteria.
Main Methods:
- Review of current proteomic protocols and bioinformatic pipelines.
- Analysis of proteomic applications for pathogen characterization (morphology, life stages, resistance, epidemiology).
- Focus on MALDI-TOF MS platforms for diagnostic microbiology.
Main Results:
- Proteomics provides detailed pathogen proteome descriptions and interaction analyses.
- Advanced applications enable individual pathogen proteome charting and resistance profiling.
- MALDI-TOF MS facilitates advanced diagnostics for emerging and fastidious pathogens.
Conclusions:
- Proteomics significantly advances the diagnosis and management of infectious diseases.
- Proteomic strategies are essential for understanding complex microbial ecosystems.
- MALDI-TOF MS platforms offer powerful tools for patient-tailored microbiological diagnostics.
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