Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cohesins02:20

Cohesins

4.5K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.5K
Cohesins02:20

Cohesins

2.1K
2.1K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.1K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

1.0K
1.0K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
Homologous Recombination02:31

Homologous Recombination

6.4K
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hypoxia Independently Induces AID Expression in CH12 B Cells.

European journal of immunology·2026
Same author

Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts.

Nature communications·2026
Same author

Real-world comparison of COBE2991 and LOVO Med devices for the post-thaw washing of peripheral blood stem cells and donor lymphocyte infusions.

Cytotherapy·2026
Same author

A Proteomic View of Butterfly Metamorphosis.

Proteomes·2025
Same author

Senataxin promotes recombination fidelity during antigen receptor gene diversification.

Science signaling·2025
Same author

Screening for Congenital Myasthenic Syndromes in Adults With Seronegative Myasthenia Gravis Using Next-Generation Sequencing.

Neurology·2025

Related Experiment Video

Updated: May 6, 2026

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
09:49

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Published on: August 13, 2010

18.4K

The cohesin complex regulates immunoglobulin class switch recombination.

Anne-Sophie Thomas-Claudepierre1, Ebe Schiavo, Vincent Heyer

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM) U964/Centre National de la Recherche Scientifique (CNRS) UMR 7104/Université de Strasbourg, 67404 Illkirch, France.

The Journal of Experimental Medicine
|October 23, 2013
PubMed
Summary

Immunoglobulin class switch recombination (CSR) relies on activation-induced cytidine deaminase (AID) and DNA repair. Cohesin complex aids CSR by recruiting AID and resolving DNA breaks, crucial for antibody diversity.

More Related Videos

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

7.5K
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

17.9K

Related Experiment Videos

Last Updated: May 6, 2026

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
09:49

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Published on: August 13, 2010

18.4K
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

7.5K
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

17.9K

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Immunoglobulin (Ig) class switch recombination (CSR) generates antibody diversity.
  • CSR involves transcription-coupled recruitment of activation-induced cytidine deaminase (AID) and DNA double-strand breaks (DSBs).
  • DSBs are repaired via nonhomologous end joining (NHEJ).

Purpose of the Study:

  • To investigate the role of the cohesin complex in Ig class switch recombination (CSR).
  • To determine if cohesin interacts with AID during CSR.
  • To elucidate cohesin's function in resolving DNA breaks during CSR.

Main Methods:

  • Co-immunoprecipitation to detect AID-cohesin association.
  • Chromatin immunoprecipitation (ChIP) to assess cohesin recruitment to the IgH locus.
  • CRISPR/Cas9 or siRNA to knock down cohesin subunits.
  • Analysis of CSR efficiency and DNA repair pathway usage.

Main Results:

  • Cohesin subunits associate with AID during CSR.
  • Cohesin is dynamically recruited to the Sμ-Cμ region of the IgH locus during CSR.
  • Knockdown of cohesin or its regulators impairs CSR.
  • Impaired CSR correlates with increased microhomology-based end joining.

Conclusions:

  • The cohesin complex plays a critical role in the mechanism of Ig class switch recombination.
  • Cohesin facilitates AID recruitment and proper resolution of DNA breaks during CSR.
  • Cohesin's function in CSR is essential for maintaining antibody diversity and preventing aberrant DNA repair.