Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Calcium channels: basic properties as revealed by radioligand binding studies.

H Glossmann, D R Ferry, A Goll

Journal of Cardiovascular Pharmacology

|January 1, 1985

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Treatment mapping of lower urinary tract symptoms due to benign prostatic hyperplasia-an analysis of the Governing Body of German Prostate Centers].

Der Urologe. Ausg. A·2020

Same author

Occupational exposure to ultrafine particles in police officers: no evidence for adverse respiratory effects.

Journal of occupational medicine and toxicology (London, England)·2018

Same author

A randomized, open-label study of the efficacy and safety of AZD4547 monotherapy versus paclitaxel for the treatment of advanced gastric adenocarcinoma with FGFR2 polysomy or gene amplification.

Annals of oncology : official journal of the European Society for Medical Oncology·2017

Same author

Enhancing VTA Ca<sub>v</sub>1.3 L-type Ca<sup>2+</sup> channel activity promotes cocaine and mood-related behaviors via overlapping AMPA receptor mechanisms in the nucleus accumbens.

Molecular psychiatry·2017

Same author

Enhancing dopaminergic signaling and histone acetylation promotes long-term rescue of deficient fear extinction.

Translational psychiatry·2016

Same author

[Treatment mapping of prostate cancer in DVPZ prostate centers in Germany].

Der Urologe. Ausg. A·2015

Researchers explored calcium channel structure using radiolabeled drugs, identifying three distinct receptor sites. Novel ligands like 125I-iodipine and 3H-azidopine aid in characterizing the channel

Area of Science:

Pharmacology
Molecular Biology
Biochemistry

Background:

Calcium channels are crucial for cellular function.
Understanding their structure is key to drug development.
Previous studies suggested multiple drug-binding sites on calcium channels.

Purpose of the Study:

To investigate the structural features and molecular properties of calcium channels.
To explore the concept of multiple drug receptor sites.
To introduce novel radioligands for calcium channel characterization.

Main Methods:

Utilized radiolabeled ligands: 1,4-dihydropyridines, d-cis diltiazem, and verapamil.
Employed radioligands 125I-iodipine, 3H-azidopine, and (-) 3H-desmethoxyverapamil.
Performed binding assays on detergent-solubilized calcium channels from skeletal muscle t-tubules.

Related Experiment Videos

Main Results:

Identified and characterized at least three distinct drug receptor sites (1, 2, and 3) on calcium channels.
Demonstrated reciprocal allosteric communication between these receptor sites.
Novel ligands successfully labeled specific sites, aiding in channel isolation and characterization.
Evidence suggests an oligomeric structure for the calcium channel, with a 145,000 Mr polypeptide identified.

Conclusions:

The calcium channel possesses multiple, allosterically communicating drug receptor sites.
Novel radioligands facilitate the isolation and detailed characterization of calcium channels.
The findings support an oligomeric model for calcium channel structure.