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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 6, 2026

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.

Shaun W Lim¹, Adam R Abate

¹UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, California, USA.

|October 23, 2013

Summary

We present a new method for sorting aqueous droplets using flow cytometry, enhancing speed and sensitivity for biological applications. This approach simplifies complex microfluidic systems for high-throughput screening.

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Last Updated: May 6, 2026

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Area of Science:

Microfluidics
Flow Cytometry
Biotechnology

Background:

Droplet detection and sorting are crucial for high-throughput biological applications like directed evolution and digital PCR.
Current methods often involve complex, costly microfluidic systems with limited sensitivity and throughput.

Purpose of the Study:

To develop a general method for droplet-based microfluidic sorting using flow cytometry.
To overcome the limitations of existing droplet sorting techniques.

Main Methods:

Harnessing the speed and sensitivity of flow cytometry for droplet sorting.
Integrating flow cytometry with droplet microfluidics.

Main Results:

Demonstrated a general method for droplet microfluidic sorting.
Achieved high-throughput sorting with enhanced speed and sensitivity.

Conclusions:

Flow cytometry offers a powerful and accessible platform for droplet-based microfluidic sorting.
This method simplifies complex systems, improving efficiency for biological screening and analysis.