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Characterization of metal-tagged antibodies used in ICP-MS-based immunoassays

L Mueller1, T Mairinger, G Hermann

  • 1BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Straße 11, 12489, Berlin, Germany, larissa.mueller@bam.de.

Analytical and Bioanalytical Chemistry
|October 23, 2013
PubMed

A detailed characterization of metal-tagged antibodies is the prerequisite for the implementation of quantitative concepts in inductively coupled plasma-mass spectrometry (ICP-MS)-based bioanalysis or future medical diagnosis. In this paper, the common modification with bifunctional ligands containing maleimide residues as a reactive group was investigated in detail via size exclusion chromatography (SEC)-ICP-MS and liquid chromatography-time-of-flight (LC-TOF)-MS to determine the preservation of the antibody structure after tagging. Mouse monoclonal IgG modified with metal-coded tags (MeCATs) was used as a model system. Several antibody fragments were identified carrying different numbers of metal tags. In a second step, a functionality test was performed with isolated fragments where the antigen specificity was tested in a dot blot immunoassay.

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