Related Experiment Video
Updated: May 6, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
The inhibitory effect of Binens bipinnata L. extract on U14 tumour in mice
Li-Hong Zhu1, Rui-Ying Qin, Hui-Min Guo
1The First Affiliated Hospital of Xinxiang Medical College University, Henan Province, China.
Abstract:
The objective of this paper was to study the in vitro and in vivo inhibitory effect of Bidens bipinnata L. extract on growth of cervical carcinoma U14 cells. MTT method was used to determine the inhibitory effect of Bidens bipinnata L. extract on U14 tumour cells, and the effects of Bidens bipinnata L. extract on inhibition rate of solid tumour and life prolongation rate of ascites tumour were observed through the establishment of two animal models of mouse cervical carcinoma U14 solid tumour and ascites tumour. In the in vitro MTT assay, the inhibition rate gradually increased with the increase of dose of Bidens bipinnata L. and the extension of time. Its inhibition rate was 70.44% at a concentration of 80µg/L. Solid tumour inhibition rates in the high- and low-dose groups and cisplatin group were 49.13%, 2.26% and 75.72% respectively; life prolongation rates in each ascites tumour group were 63.63%, 34.86% and 87.34% respectively. The Bidens bipinnata L. extract has a certain inhibitory effect on growth of mouse cervical carcinoma U14.
Insights
Bidens bipinnata L. extract shows significant potential in inhibiting cervical carcinoma U14 cell growth. Both in vitro and in vivo studies demonstrated its anti-tumor effects, suggesting therapeutic possibilities.
Area of Science:
- Phytochemistry
- Oncology
- Pharmacology
Background:
- Cervical carcinoma remains a significant global health challenge.
- Exploring natural compounds for cancer treatment is crucial.
Purpose of the Study:
- To investigate the anti-cancer properties of Bidens bipinnata L. extract.
- To evaluate its inhibitory effects on cervical carcinoma U14 cells in vitro and in vivo.
Main Methods:
- In vitro MTT assay to assess cytotoxicity against U14 cells.
- In vivo studies using mouse models for solid and ascites tumors.
- Measurement of solid tumor inhibition and ascites tumor life prolongation rates.
Main Results:
- Bidens bipinnata L. extract demonstrated dose- and time-dependent inhibition of U14 cells in vitro, reaching 70.44% at 80µg/L.
- In vivo, high-dose extract achieved a 49.13% solid tumor inhibition rate and 63.63% life prolongation rate.
- Cisplatin showed higher efficacy (75.72% solid tumor inhibition, 87.34% life prolongation).
Conclusions:
- Bidens bipinnata L. extract exhibits a notable inhibitory effect on the growth of cervical carcinoma U14.
- Further research is warranted to explore its therapeutic potential and mechanisms of action.
More Related Videos
09:03Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula
Published on: January 26, 2024
11:06Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
Published on: May 20, 2015