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Updated: May 6, 2026
Development and Standardization of an Ex Vivo Micromethod for Intracellular Quantification of Vincristine in Primary ALL Cells by LC-MS/MS
Published on: January 23, 2026
Determination of vinblastine in tumour tissue with liquid chromatography-high resolution mass spectrometry
Tina Kosjek1, Tanja Dolinšek, Darja Gramec
1Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
Abstract:
There are virtually no analytical methods that describe determination of vinblastine and other vinca alkaloids in tumour tissue, albeit quantitative data on tumour drug amount is essential for maximal benefit of a particular anticancer treatment. The analytical method presented herein uses state-of-the-art sample preparation, separation and detection techniques to allow sensitive and selective determination of vinblastine in tumour tissue. After cryogenic grinding and sonication, tumour suspensions were extracted by Oasis MAX solid phase extraction and analysed for vinblastine with ultra-high performance liquid chromatography coupled to positive electrospray ionisation-high resolution mass spectrometric detection. The developed analytical method quantifies vinblastine down to 23 ng/g tumour tissue and shows satisfactory linearity (r(2)>0.99), precision (1.1-8.2%), accuracy (98%) and high selectivity with almost complete absence of matrix effects. The proposed method was found suitable to follow vinblastine levels in mice tumours and could be used to support preclinical pharmacologic studies.
Insights
A new analytical method accurately measures vinblastine in tumor tissue, crucial for optimizing cancer treatment. This technique enhances precision and selectivity for better preclinical drug studies.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Oncology
Background:
- Quantitative analysis of vinblastine in tumor tissue is lacking.
- Accurate drug quantification is essential for effective anticancer therapy.
- Vinca alkaloids are vital anticancer agents requiring precise measurement.
Purpose of the Study:
- To develop a sensitive and selective analytical method for vinblastine determination in tumor tissue.
- To enable precise quantification of vinblastine for preclinical pharmacologic studies.
- To address the need for robust analytical techniques in cancer drug research.
Main Methods:
- Cryogenic grinding and sonication for sample preparation.
- Oasis MAX solid-phase extraction for analyte isolation.
- Ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) for detection.
Main Results:
- The method quantifies vinblastine down to 23 ng/g in tumor tissue.
- Demonstrated high linearity (r(2)>0.99), precision (1.1-8.2%), and accuracy (98%).
- Exhibited excellent selectivity with minimal matrix effects.
Conclusions:
- The developed method provides sensitive and selective vinblastine quantification in tumor tissue.
- Suitable for monitoring vinblastine levels in preclinical cancer studies.
- Supports optimization of anticancer treatment strategies through accurate drug quantitation.
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