Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Cell Death01:30

Overview of Cell Death

7.8K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.8K
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

121
Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
121
Apoptosis01:30

Apoptosis

12.3K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
Replicative Cell Senescence02:15

Replicative Cell Senescence

3.5K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthetic Small Molecules as Regulators of In Vitro Multiplication in <i>Selenicereus</i> Hybrids.

Plants (Basel, Switzerland)·2026
Same author

Epigenetic remodeling during UV exposure: high resolution analysis of histone post-translational modifications in a DNA binding protein 2 mutant model.

Histochemistry and cell biology·2026
Same author

An endogenous viral element of Aedes albopictus is translated and limits cognate virus.

BMC biology·2026
Same author

Resolving Sub-Nuclear Architecture from Compartments to Functional Domains.

International journal of molecular sciences·2026
Same author

Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.

Plant molecular biology·2026
Same author

Left bundle branch area vs biventricular pacing for cardiac resynchronization therapy: the LEFT-BUNDLE-CRT trial.

European heart journal·2026

Related Experiment Video

Updated: May 6, 2026

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
12:44

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Published on: October 11, 2012

21.8K

Epigenetic changes accompany developmental programmed cell death in tapetum cells.

María-Teresa Solís1, Nandini Chakrabarti, Eduardo Corredor

  • 1Pollen Biotechnology of Crop Plants group, Biological Research Center, CIB, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

Plant & Cell Physiology
|October 24, 2013
PubMed
Summary

Programmed cell death (PCD) in plant tapetum involves increased DNA methylation and MET1 expression, suggesting a novel epigenetic role in regulating nuclear changes during this crucial developmental process.

Keywords:
5-MethyldeoxycytosineAnther developmentBrassica napusDNA methylationDNA methyltransferaseNicotiana tabacum

More Related Videos

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
07:50

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Published on: August 29, 2018

10.0K
Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

17.9K

Related Experiment Videos

Last Updated: May 6, 2026

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
12:44

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Published on: October 11, 2012

21.8K
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
07:50

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Published on: August 29, 2018

10.0K
Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

17.9K

Area of Science:

  • Plant reproductive biology
  • Epigenetics
  • Cellular biology

Background:

  • The tapetum, essential anther tissue, undergoes programmed cell death (PCD) during pollen development.
  • Molecular mechanisms and epigenetic regulation of tapetal PCD are poorly understood.
  • DNA methylation, mediated by DNA methyltransferases like MET1, is a key epigenetic mechanism.

Purpose of the Study:

  • To investigate epigenetic changes, specifically DNA methylation dynamics and MET1 expression, during tapetal PCD.
  • To correlate these epigenetic modifications with cellular events in tapetal PCD.
  • To explore the role of epigenetic marks in nuclear events during plant PCD.

Main Methods:

  • Multidisciplinary approach to study epigenetic marks in tapetal PCD.
  • Analysis of DNA methylation dynamics and MET1 gene expression.
  • Characterization of nuclear architecture, chromatin condensation, and cell death markers (caspase 3-like proteases, Cyt c release).
  • Comparative study in Brassica napus and Nicotiana tabacum.

Main Results:

  • Tapetal PCD is associated with increased global DNA methylation.
  • MET1 expression significantly increases during tapetal PCD.
  • Epigenetic changes correlate with nuclear reorganization, chromatin condensation, and markers of apoptosis.
  • Observed dynamics in Brassica napus and Nicotiana tabacum.

Conclusions:

  • A relationship exists between tapetal PCD, DNA methylation dynamics, and MET1 expression.
  • Epigenetic marks may play a novel role in the nuclear events of tapetal PCD.
  • Findings provide new insights into the epigenetic control of programmed cell death in plants.