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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Statistical optimization of alkaline protease production from brackish environment Bacillus sp. SKK11 by SSF using
Muthusamy Govarthanan1, Sung-Hee Park, Jin-Won Kim
1a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
Abstract:
Protease production by Bacillus sp. SKK11 isolated from brackish environment was studied by solid-state fermentation with horse gram husk. Response surface methodology-based Box-Behnken design (BBD) was used to optimize the variables such as pH, maltose, and MgSO₄. The BBD design analysis showed a reasonable adjustment of the quadratic model with the experimental data. Statistics-based contour and three-dimensional (3-D) plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions. The maximum yield of the enzyme was observed at pH 9.0.
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