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Updated: Aug 8, 2026

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
11:00

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Published on: September 10, 2010

[Decrease in the transforming action of Rous sarcoma virus produced by avian cells grown in the presence of

F Presse, F Lawrence, A Pierré

    Comptes Rendus De L'Academie Des Sciences. Serie III, Sciences De La Vie
    |January 1, 1985
    PubMed

    Insights

    The drug 5'-deoxy-5'-S-isobutyladenosine (SIBA) significantly inhibits Rous Sarcoma virus production in chick cells. SIBA treatment yields non-infective viral particles, impacting viral transformation and replication.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Context:

    • Rous Sarcoma virus (RSV) is a retrovirus that causes tumors in chickens.
    • Chick embryo fibroblasts are commonly used model systems for studying viral replication and transformation.
    • 5'-deoxy-5'-S-isobutyladenosine (SIBA) is an adenosine analog with potential antiviral properties.

    Purpose:

    • To investigate the effect of SIBA on Rous Sarcoma virus production and virion characteristics.
    • To determine the mechanism by which SIBA inhibits viral replication.

    Summary:

    • Treatment of RSV-transformed chick embryo fibroblasts with 1 mM SIBA for 24 hours resulted in a 165-fold inhibition of transforming virus production.
    • Viral particle production was only inhibited 2-fold, indicating the produced virions were non-infective or non-transforming.
    • SIBA treatment led to decreased virion density, reduced levels of protein P19, and accumulation of high molecular weight proteins.

    Impact:

    • SIBA effectively inhibits the production of infectious and transforming Rous Sarcoma virus.
    • The drug alters virion composition, suggesting a disruption in viral assembly or maturation.
    • These findings provide insights into potential therapeutic strategies targeting viral replication and transformation.

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