Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular cloning of cDNA coding for human preprourokinase.

M Nagai, R Hiramatsu, T Kanéda

    Gene
    |January 1, 1985
    PubMed
    Summary

    Researchers cloned human kidney cell cDNA to produce urokinase, a key protein. This involved gene sequencing and expression in frog oocytes, yielding a 2250-bp DNA sequence.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    INVESTIGATION OF A PRACTICAL PATIENT DOSE INDEX FOR ASSESSMENT OF PATIENT ORGAN DOSE FROM CONE-BEAM COMPUTED TOMOGRAPHY IN RADIATION THERAPY USING A MONTE CARLO SIMULATION.

    Radiation protection dosimetry·2018
    Same author

    SU-E-J-26: Automated Estimation Method of Patient Setup Errors Using Simulated Portal Images for Prostate Cancer Radiotherapy.

    Medical physics·2017
    Same author

    SU-E-I-92: Accuracy Evaluation of Depth Data in Microsoft Kinect.

    Medical physics·2017
    Same author

    SU-E-I-91: Development of a Compact Radiographic Simulator Using Microsoft Kinect.

    Medical physics·2017
    Same author

    TU-E-BRB-01: Similar-Case-Based Optimization of Beam Arrangements in Stereotactic Body Radiation Therapy.

    Medical physics·2017
    Same author

    WE-G-BRCD-04: Development of Automated Determination Method of Robust Beam Directions against Patient Setup Errors Based on Spatial Distribution of Electron Density in Particle Therapy.

    Medical physics·2017

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Urokinase is a crucial enzyme in fibrinolysis.
    • Understanding urokinase gene structure is vital for therapeutic applications.

    Purpose of the Study:

    • To clone and characterize the cDNA encoding human urinary urokinase.
    • To enable the production and study of urokinase.

    Main Methods:

    • Extraction and fractionation of mRNA from human kidney cells.
    • Construction of a cDNA library in pBR322.
    • Hybridization using synthetic oligonucleotide probes based on known amino acid sequences.
    • Expression confirmation in Xenopus laevis oocytes.

    Main Results:

    Related Experiment Videos

  • A 2250-bp cDNA sequence was cloned.
  • The sequence codes for a 431-amino acid preprourokinase, including a 20-residue signal peptide.
  • Identified 5' and 3' untranslated regions of at least 80 bp and over 850 bp, respectively.

    • Conclusions:

    • Successfully cloned and characterized the human urokinase precursor cDNA.
    • The cloned cDNA provides a basis for further studies and potential biotechnological applications of urokinase.