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Proliferation of parenchymal microglia is the main source of microgliosis after ischaemic stroke

Ting Li1, Shengru Pang, Yongping Yu

  • 1School of Life Sciences, Lanzhou University, Lanzhou 73000, China.

Brain : a Journal of Neurology
|October 25, 2013
PubMed

Insights

Stroke causes microgliosis, but resident microglia, not blood cells, are the main source. Resident microglia proliferate near the stroke core, while blood-derived cells infiltrate temporarily and then undergo apoptosis.

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Microglia activation and expansion are rapid responses to stroke.
  • The primary source of cells contributing to microgliosis following stroke remains debated.

Purpose of the Study:

  • To investigate the origins of microgliosis and circulating cell infiltration after stroke.
  • To differentiate between resident microglia proliferation and blood-derived cell contributions.

Main Methods:

  • Utilized a photothrombosis stroke model in mice.
  • Employed parabiosis and in vivo two-photon microscopy for cellular tracking.
  • Conducted immunohistology and apoptosis assays.

Main Results:

  • A small population of blood-derived CX3CR1(GFP/+) cells infiltrated the brain but did not proliferate.
  • Infiltrating blood cells showed distinct kinetics, peaking at Day 5 and undergoing apoptosis.
  • Resident microglia proliferated and were continuously recruited to the ischemic area, forming the primary source of microgliosis.

Conclusions:

  • Microgliosis in stroke is predominantly driven by resident microglia proliferation.
  • Blood-derived infiltrating cells and reactive microglia are distinct populations with different roles and therapeutic potential in stroke.
  • Demonstrated active microglial division surrounding the ischemic core in vivo.
Keywords:
infiltrating cellsmicrogliamicrogliosisparabiosisstroke

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