Related Experiment Video

Updated: May 6, 2026

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
07:39

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

Published on: June 25, 2018

6.8K

Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells

Young Rae Kim1, Jong Seok Lee, Ki Rim Lee

  • 1Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Kangwon‑do 200‑701, Republic of Korea.

International Journal of Molecular Medicine
|October 25, 2013
PubMed

Insights

Mulberry extracts demonstrate significant antioxidant and cytoprotective effects in pancreatic beta cells. These findings suggest mulberry

Area of Science:

  • Oxidative stress and cellular damage
  • Diabetes research
  • Natural product therapeutics

Background:

  • Reactive oxygen species (ROS) are implicated in cellular damage and diseases like type 1 diabetes.
  • Type 1 diabetes involves the destruction of pancreatic beta cells, partly due to ROS activity.

Purpose of the Study:

  • To investigate the cytoprotective and antioxidant effects of fractionated mulberry extracts.
  • To assess the impact of mulberry extracts on mouse insulin-producing pancreatic beta cells (MIN6N cells) under oxidative stress.

Main Methods:

  • MIN6N cells were treated with hydrogen peroxide (H2O2) to induce oxidative stress.
  • The effects of fractionated mulberry extracts on cell death, intracellular ROS, DPPH radical scavenging, lipid peroxidation, and DNA fragmentation were evaluated.

Main Results:

  • Hydrogen peroxide (H2O2) treatment caused significant cell death, increased ROS, lipid peroxidation, and DNA fragmentation in MIN6N cells.
  • Fractionated mulberry extracts markedly reduced H2O2-induced intracellular ROS, DPPH radicals, and lipid peroxidation.
  • Mulberry extracts effectively inhibited H2O2-induced DNA fragmentation in pancreatic beta cells.

Conclusions:

  • Mulberry extracts possess significant antioxidant properties beneficial for pancreatic beta cells.
  • These antioxidant properties suggest potential applications of mulberry extracts in preventing or treating type 1 diabetes.

More Related Videos

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds12:32

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Published on: January 23, 2018

10.8K
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes11:03

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Published on: September 9, 2021

3.3K

Related Concept Videos

Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
13.7K
Chronic Pancreatitis II: Pathophysiology01:21

Chronic Pancreatitis II: Pathophysiology

Chronic pancreatitis is a progressive and irreversible inflammation of the pancreas, most often caused by long-term alcohol abuse, but it can also be related to ductal obstruction, smoking, or genetic factors.Chronic pancreatitis occurs when the pancreas is repeatedly exposed to harmful agents like alcohol, smoking, ductal obstruction, or genetic predisposition. These factors lead to the release of toxic metabolites and inflammatory cytokines, sustaining chronic inflammation in the pancreatic...
36