Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inclusive Search for Anomalous Single-Photon Production in MicroBooNE.

Physical review letters·2026
Same author

First Search for Dark Sector e^{+}e^{-} Explanations of the MiniBooNE Anomaly at MicroBooNE.

Physical review letters·2026
Same author

In vivo evaluation of the anti-inflammatory efficacy of liposomes loaded with Echinacea purpurea roots extracts.

Drug delivery and translational research·2026
Same author

A two-mode thermomechanically squeezed phonon laser.

Nature communications·2026
Same author

First Measurement of Charged-Current Muon-Neutrino-Induced K^{+} Production on Argon Using the MicroBooNE Detector.

Physical review letters·2026
Same author

Evaluation of Cytocompatibility and Anti-Inflammatory Activity of Carboxyxanthones Selected by In Silico Studies.

International journal of molecular sciences·2026

Related Experiment Video

Updated: May 6, 2026

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
07:51

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

8.4K

Conditioned medium as a strategy for human stem cells chondrogenic differentiation.

M L Alves da Silva1,2, A R Costa-Pinto1,2, A Martins1,2

  • 13B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.

Journal of Tissue Engineering and Regenerative Medicine
|October 25, 2013
PubMed
Summary

Chondrocyte conditioned medium effectively promotes stem cell chondrogenesis without growth factors. Wharton

Keywords:
cartilagechondrogenic differentiationconditioned mediumstem cells

More Related Videos

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
08:30

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Published on: February 24, 2017

9.0K
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
14:46

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Published on: January 20, 2018

6.8K

Related Experiment Videos

Last Updated: May 6, 2026

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
07:51

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

8.4K
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
08:30

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Published on: February 24, 2017

9.0K
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
14:46

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Published on: January 20, 2018

6.8K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Chondrocytes secrete factors that enhance cartilage extracellular matrix (ECM) synthesis by stem cells.
  • Using chondrocyte-conditioned medium offers a potential clinical strategy to stimulate stem cell chondrogenesis, possibly reducing reliance on donor chondrocytes.

Purpose of the Study:

  • To investigate the potential of conditioned medium from human articular chondrocytes to induce chondrogenic differentiation in human bone marrow-derived mesenchymal stem cells (hBMSCs) and human Wharton's jelly-derived stem cells (hWJSCs).
  • To evaluate this differentiation process without the addition of exogenous growth factors.
  • To assess the efficacy of a chitosan:poly(butylene succinate) scaffold in supporting this differentiation.

Main Methods:

  • Human articular chondrocyte-conditioned medium was used to culture hBMSCs and hWJSCs.
  • Stem cells were cultured within 3D scaffolds made of a 50:50 chitosan:poly(butylene succinate) blend.
  • Chondrogenic differentiation was assessed by measuring glycosaminoglycans (GAGs) accumulation and the expression of cartilage-related genes (aggrecan, Sox9, collagen type II).

Main Results:

  • Both hBMSCs and hWJSCs successfully underwent chondrogenic differentiation when cultured in chondrocyte-conditioned medium on the 3D scaffolds, without added growth factors.
  • hWJSCs demonstrated significantly higher GAGs accumulation and expression of key cartilage genes compared to hBMSCs.
  • The conditioned medium successfully induced MSC chondrogenesis and ECM formation.

Conclusions:

  • Articular chondrocyte-conditioned medium is a potent, growth factor-free stimulus for inducing chondrogenic differentiation of MSCs on chitosan:poly(butylene succinate) scaffolds.
  • hWJSCs show superior chondrogenic potential compared to hBMSCs under these conditions.
  • This approach represents a promising strategy for clinical applications in cartilage tissue engineering.