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Corneal mineralization in wistar hannover rats
Satomi Hashimoto1, Takuya Doi, Yumi Wako
1Pathology Department, Kashima Laboratory, Nonclinical Research Center, Mitsubishi Chemical Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan.
Journal of Toxicologic Pathology
|October 25, 2013
Summary
Wistar Hannover rats frequently exhibit corneal mineralization, a condition confirmed to involve calcium deposits. This incidence is higher in males than females, differing from Sprague-Dawley rats.
Area of Science:
- Ophthalmology
- Veterinary Pathology
- Rodent Models
Background:
- Wistar Hannover rats are increasingly used in Japan for research.
- Frequent observations of corneal mineralization in RccHan(TM):WIST and Crl:WI (Han) strains prompted this investigation.
- Background data collection is ongoing for this rat model.
Purpose of the Study:
- To histopathologically and ultrastructurally investigate corneal mineralization in Wistar Hannover rats.
- To compare the incidence of corneal mineralization in Wistar Hannover rats with Sprague-Dawley rats.
- To determine the characteristics and potential cellular responses to corneal mineralization.
Main Methods:
- Histopathological examination of rat corneas.
- Ultrastructural investigation of corneal tissues.
- Von Kossa's staining method to detect calcium deposits.
Main Results:
- Wistar Hannover rats showed a significantly higher incidence of corneal mineralization compared to Sprague-Dawley rats.
- Corneal mineralization was more prevalent in male Wistar Hannover rats than in females.
- Mineral deposits tested positive for calcium using von Kossa's method.
- Keratocytes appeared to become active in response to the mineralization.
Conclusions:
- Corneal mineralization is a common finding in Wistar Hannover rats, particularly in males.
- The mineralization involves calcium deposits and elicits a cellular response from keratocytes.
- Further research may explore the mechanisms and implications of this condition in Wistar Hannover rats.
