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Circulating progenitor and mature endothelial cells in deep vein thrombosis

Aline M Alessio1, Miriam P Beltrame, Mariane C Flores Nascimento

  • 11. Hematology and Hemotherapy Center, University of Campinas, Campinas-SP, Brazil.

International Journal of Medical Sciences
|October 25, 2013
PubMed

Insights

Circulating endothelial cells (CEC) and progenitor cells (EPC) increase after deep vein thrombosis (DVT). These cells may play a role in DVT repair, with distinct release patterns observed in experimental and human studies.

Area of Science:

  • Vascular Biology
  • Hematology
  • Cellular Biology

Background:

  • Mature circulating endothelial cells (CEC) and circulating endothelial progenitor cells (EPC) are linked to endothelial injury.
  • The involvement of CEC and EPC in deep vein thrombosis (DVT) remains unevaluated.

Purpose of the Study:

  • To investigate the time course of CEC and EPC release following experimental DVT in mice.
  • To assess the presence of CEC and EPC in patients with DVT during acute and chronic phases.

Main Methods:

  • Utilized the FeCl3 model for experimental DVT induction in mice.
  • Employed Flow Cytometry to quantify CEC and EPC levels in both murine models and human DVT patients.
  • Analyzed cell counts in acute and chronic DVT patient phases, as well as serial samples over time.

Main Results:

  • In mice, CEC and EPC levels elevated 24 hours post-DVT induction, peaking at 48 hours.
  • CEC counts declined sharply after 72 hours, while EPC counts showed a less substantial decrease.
  • DVT patients exhibited significantly increased CEC counts during both acute and chronic phases compared to healthy controls.

Conclusions:

  • Findings suggest CEC and EPC participate in DVT repair processes at both early and late stages.
  • Differential kinetics of CEC and EPC release indicate distinct roles in post-DVT reparative events.
  • Elevated CEC levels in chronic DVT patients warrant further investigation into their long-term implications.
Abstract

Keywords:
DVT animal model.deep vein thrombosisflow cytometrymature endothelial cellsprogenitor endothelial cells

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