Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.8K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

919
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
919
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Widefield pump-probe microscopy with coherent background subtraction by angle-compensated temporal shearing.

Optics express·2026
Same author

Overcoming the Indirect Band Gap: Efficient Silicon Emission via Momentum-Engineered Photonic States.

Nano letters·2026
Same author

Selective Visualization of Type II Collagen Using Sum-Frequency Generation (SFG).

Journal of biophotonics·2026
Same author

Subthreshold membrane depolarization powerfully engages intracellular calcium dynamics in the brain.

bioRxiv : the preprint server for biology·2026
Same author

Implications of temporal sampling in voltage imaging microscopy.

ArXiv·2026
Same author

Relative phase of membrane potential theta oscillations between individual hippocampal neurons code space.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: May 6, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.0K

Introduction to the Novel Techniques in Microscopy feature issue.

Jerome Mertz1, Eric O Potma

  • 1Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

Biomedical Optics Express
|October 25, 2013
PubMed
Summary

This feature issue highlights novel microscopy techniques discussed at a 2013 symposium. The event focused on advancements in optical imaging for life sciences research.

Keywords:
(000.1200) Announcements, awards, news, and organizational activities(180.0180) Microscopy

More Related Videos

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
11:48

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Published on: May 17, 2022

4.9K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.0K

Related Experiment Videos

Last Updated: May 6, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.0K
Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
11:48

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Published on: May 17, 2022

4.9K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.0K

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Life Sciences

Background:

  • Microscopy is crucial for visualizing biological structures.
  • Advancements in microscopy drive biological discovery.
  • The Optics in the Life Sciences Congress convenes researchers.

Purpose of the Study:

  • To introduce a special issue on novel microscopy techniques.
  • To summarize key developments presented at a related symposium.
  • To foster discussion on the future of biological imaging.

Main Methods:

  • The content is based on presentations from a symposium.
  • The symposium focused on novel microscopy techniques.
  • The feature issue compiles selected contributions.

Main Results:

  • The symposium showcased innovative microscopy approaches.
  • Discussions covered advancements in optical microscopy.
  • New techniques offer enhanced biological visualization.

Conclusions:

  • Novel microscopy techniques are rapidly evolving.
  • These advancements are critical for progress in life sciences.
  • Continued innovation in imaging is expected.