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A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
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Simple, miniaturized blood plasma extraction method.

Jin-Hee Kim1, Timothy Woenker, Jiri Adamec

  • 1Novilytic Laboratories , West Lafayette, Indiana 47906, United States.

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|October 26, 2013
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A new plasma extraction technology allows rapid, finger-stick blood collection on cards, simplifying sample transport and analysis for biomarkers like 25-Hydroxy vitamin D and proteins.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Traditional blood collection methods (venipuncture) require specialized equipment and trained personnel.
  • Sample transport and storage often necessitate refrigeration, adding logistical challenges.
  • Developing point-of-care or simplified blood collection methods is crucial for wider accessibility.

Purpose of the Study:

  • To evaluate a novel, rapid plasma extraction technology using finger-stick blood.
  • To assess the suitability of dried plasma samples for downstream analytical measurements.
  • To compare the performance of this new method with conventional venipuncture-based assays.

Main Methods:

  • A 2.5 μL plasma aliquot was collected within three minutes from a finger-stick blood drop using a membrane stack and capillary action.
  • Plasma was absorbed onto a paper collection disc, air-dried, and then extracted for analysis.
  • Quantification of 25-Hydroxy vitamin D by LC-MS/MS and protein analysis (yeast enolase) using spectral counting were performed.

Main Results:

  • The technology demonstrated high plasma volume consistency (±1% intercard, <2% variation with hematocrit 20-71%).
  • 25-Hydroxy vitamin D quantification achieved a low limit of quantification (LOQ) of 1 pg, comparable to venipuncture methods.
  • Protein quantification (enolase) showed comparable results to venipuncture samples, with an LOQ of 1 μM.

Conclusions:

  • This rapid plasma extraction technology offers a viable alternative to venipuncture for collecting blood samples.
  • Dried plasma samples are suitable for accurate quantification of various biomarkers, including vitamins and proteins.
  • The method simplifies sample handling, transport, and analysis, potentially enabling decentralized diagnostics.