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Effects of retinoic acid isomers on proteomic pattern in human breast cancer MCF-7 cell line
Abstract:
Retinoids, acting through their cognate nuclear receptors, are crucial transcriptional regulators of many cellular processes such as differentiation, development, apoptosis, carbohydrate and lipid metabolism, homeostasis, etc. The aim of this study was the exploration of molecular mechanisms in relation to therapy of human breast cancer. One of the efficient strategies is identification of biomarkers as important tools in early cancer diagnosis and advisable treatment. Retinoids have been regarded as important therapeutic agents for many types of cancers, including human breast cancer. The effects of all-trans retinoic acid and 9-cis retinoic acid or their combination on proteomic pattern in human MCF-7 breast cancer line were investigated. The total cell proteins were extracted utilizing a commercially Radio-Immunoprecipitation Assay (RIPA) buffer and separated on 1D sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE). The proteins were subsequently digested in-gel by trypsin and identified by matrix assisted laser desorption ionization technique with time of flight mass analyzer (MALDI-TOF/TOF). Our data offer novel information on the proteomic pattern of proteins evaluated after treatment of MCF-7 cells with retinoic acid isomers.
Insights
Retinoids regulate cellular processes and are vital for breast cancer therapy. This study investigated how all-trans retinoic acid and 9-cis retinoic acid impact the proteomic patterns in human MCF-7 breast cancer cells.
Area of Science:
- Molecular Biology
- Cancer Research
- Proteomics
Background:
- Retinoids are critical transcriptional regulators involved in numerous cellular functions.
- Retinoids show therapeutic potential in various cancers, including breast cancer.
- Identifying biomarkers is key for early cancer diagnosis and effective treatment strategies.
Purpose of the Study:
- To explore the molecular mechanisms of retinoid therapy in human breast cancer.
- To investigate the effects of all-trans retinoic acid and 9-cis retinoic acid on the proteomic profile of MCF-7 breast cancer cells.
Main Methods:
- Proteins were extracted from MCF-7 cells using Radio-Immunoprecipitation Assay (RIPA) buffer.
- Proteins were separated using 1D sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE).
- In-gel digested proteins were identified using matrix assisted laser desorption ionization time of flight mass analyzer (MALDI-TOF/TOF).
Main Results:
- The study identified changes in the proteomic pattern of MCF-7 cells after treatment with retinoic acid isomers.
- Novel information on protein expression was obtained following treatment with all-trans retinoic acid and 9-cis retinoic acid.
Conclusions:
- Retinoic acid isomers alter the proteomic landscape of human breast cancer cells.
- These findings contribute to understanding retinoid mechanisms in breast cancer therapy.
