Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

167
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
167
Gene Therapy00:59

Gene Therapy

22.3K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
22.3K
Bioplastics01:27

Bioplastics

73
Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
73
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.4K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.4K
Recombinant DNA01:09

Recombinant DNA

92.9K
Overview
92.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery and Cryo-EM-Guided Development of a Neuropilin-2-Binding Aptamer for Receptor Antagonism.

Journal of the American Chemical Society·2026
Same author

Peptide vaccine formulations with structurally distinct STING agonist drugamers induce discrete, efficacious antitumor responses.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Rapid and label-free isolation of human peripheral blood monocytes using a reversible CD36-binding aptamer for cell capture.

Biomaterials science·2025
Same author

Investigating PolySTAT's role in clot contraction and fibrin network mechanics.

Biomaterials science·2025
Same author

Efficient and Traceless Aptamer-Based Serial Selection of Naïve and Early Memory CD8 T Cells for CAR T Cell Therapy.

Advanced healthcare materials·2025
Same author

Lytic polyplex vaccines enhance antigen-specific cytotoxic T cell response through induction of local cell death.

Advanced therapeutics·2025

Related Experiment Video

Updated: May 6, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

13.1K

Engineering biodegradable and multifunctional peptide-based polymers for gene delivery.

Julie Shi, Joan G Schellinger, Suzie H Pun1

  • 1Department of Bioengineering and Molecular Engineering & Sciences Institute, University of Washington, 3720 15th Ave NE, Seattle, WA 98195, USA. spun@uw.edu.

Journal of Biological Engineering
|October 26, 2013
PubMed
Summary

Researchers developed modular peptide-based polymers for gene delivery. This approach offers reproducible, scalable materials with tunable properties for enhanced in vivo gene transfer applications.

More Related Videos

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
09:01

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

10.6K
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

15.6K

Related Experiment Videos

Last Updated: May 6, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

13.1K
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
09:01

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

10.6K
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

15.6K

Area of Science:

  • Polymer Chemistry
  • Biomaterials Science
  • Gene Therapy

Background:

  • In vivo gene transfer requires sophisticated delivery vectors to overcome biological barriers.
  • Clinical translation of synthetic gene delivery systems necessitates reproducible and scalable manufacturing processes.

Purpose of the Study:

  • To review the development of a modular synthetic approach for creating peptide-based polymers for gene delivery.
  • To highlight the synthesis of well-defined polymers with tunable composition and molecular weight.

Main Methods:

  • Utilizing reversible-addition fragmentation chain-transfer (RAFT) polymerization to copolymerize bioactive peptides with an N-(2-hydroxypropyl)methacrylamide (HPMA) backbone.
  • Investigating the impact of various bioactive peptides and polymer architectures on gene delivery efficiency.

Main Results:

  • Demonstrated successful synthesis of well-defined, narrowly-disperse peptide-based polymers.
  • Showcased controllable polymer composition and molecular weight achievable through the modular approach.
  • Evaluated the efficacy of different peptides for DNA condensation, endosomal escape, cell targeting, and degradability.

Conclusions:

  • The modular synthesis of peptide-based polymers offers a promising strategy for developing advanced gene delivery vectors.
  • This approach facilitates the creation of reproducible and scalable materials for potential clinical applications in gene therapy.