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Updated: May 6, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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FAD C(4a)-hydroxide stabilized in a naturally fused styrene monooxygenase

Dirk Tischler1, Michael Schlömann, Willem J H van Berkel

  • 1Interdisciplinary Ecological Center, TU Bergakadmie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany; Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands; Department of Chemistry and Biochemistry, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, United States.

FEBS Letters
|October 26, 2013
PubMed

Keywords:
Catalytic mechanismFAD (C4a)-hydroperoxide and FAD C(4a)-hydroxideFAD(ox) and FAD(red)FADH(OOH) and FADH(OH)FlavoproteinMonooxygenaseRhodococcus opacus 1CPSMOStyA and StyBStyA2BStyrene epoxidationepoxidase and reductase subunit of conventional two-component SMOsnaturally fused self-sufficient SMO comprising reductase (B) and epoxidase (A2)oxidized and reduced FADstyrene monooxygenase

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