Related Experiment Video

Updated: May 6, 2026

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Published on: December 27, 2013

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Neutralisation of specific surface carboxylates speeds up translocation of botulinum neurotoxin type B enzymatic

Marco Pirazzini1, Tina Henke, Ornella Rossetto

  • 1Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Università di Padova, Viale Ugo Bassi 58/B, 35131 Padova, Italy.

FEBS Letters
|October 26, 2013
PubMed

Keywords:
BafA1Bafilomycin A1BoNT/XBotulinum neurotoxinC-terminal domain of the H(C)-fragmentC-terminal half of HCCGNsFBSH(C)H(CC)H(CN)H(N)HCLCMEMMPNN-terminal domain of the H(C)-fragmentN-terminal half of HCPBSPC12Phrenic nerve hemidiaphragm toxicity testSNAP-25SNAREsSV2TMTranslocationVAMP-2WTa pheochromocytoma cell linebafilomycin A1botulinum neurotoxin/serotype Xcerebellar granule neuronsfoetal bovine serumheavy chainlight chainmice phrenic nerveminimum essential mediumpH sensorphosphate buffered salinesoluble N-ethyl maleimide sensitive factor attachment protein receptorssynaptic vesicle (glyco-)protein 2synaptosomal-associated protein of 25kDatriple mutantvesicle associated membrane protein 2wild-type

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