Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Antimicrobial Peptide-Antibiotic Combination Treatment for Tackling Ocular and Systemic <i>Staphylococcus aureus</i> Infections.

International journal of molecular sciences·2026
Same author

The combined use of in vivo confocal microscopy and optical coherence tomography in monitoring corneal infections.

Eye (London, England)·2026
Same author

Aqueous flare dynamics following penetrating, anterior lamellar, and endothelial corneal transplantation: Quantitative comparison and early complication detection.

Eye (London, England)·2026
Same author

Optical coherence tomography features of the graft-host junctions after deep anterior lamellar keratoplasty: Long-term changes.

Indian journal of ophthalmology·2025
Same author

Amniotic Membrane in Excimer Laser Surface Ablation (ELSA): Mechanisms for Haze Prevention and Broader Ocular Surface Optimisation.

Ophthalmology and therapy·2025
Same author

Surgical Approaches for the Management of Peripheral Ulcerative Keratitis With Corneal Perforation.

Cornea·2025

Related Experiment Video

Updated: May 6, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.3K

Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies.

Bina B Kulkarni, Desmond G Powe, Andrew Hopkinson

  • 1Division of Ophthalmology and Visual Sciences, B-Floor, Eye & ENT Building, Queen's Medical Centre, Derby Road, Nottingham, UK. Harminder.Dua@nottingham.ac.uk.

BMC Ophthalmology
|October 29, 2013
PubMed
Summary

Laser microdissection effectively collects ocular surface epithelial samples for gene expression studies. This optimized protocol enhances RNA yield for microarray analysis, overcoming previous limitations in sample quantity and purity.

More Related Videos

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

6.5K
Microdissection of the Rodent Eye
11:03

Microdissection of the Rodent Eye

Published on: April 21, 2023

6.5K

Related Experiment Videos

Last Updated: May 6, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.3K
Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

6.5K
Microdissection of the Rodent Eye
11:03

Microdissection of the Rodent Eye

Published on: April 21, 2023

6.5K

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genomics

Background:

  • Collecting sufficient, uncontaminated ocular surface (OS) epithelial samples for in situ transcriptional profiling is challenging due to the microscopic nature of the tissue.
  • Adjacent tissues often contaminate samples, hindering accurate gene expression studies.

Purpose of the Study:

  • To establish an optimized protocol for collecting ocular surface epithelial tissue samples for gene expression analysis.
  • To overcome challenges in obtaining adequate sample amounts and purity for microarray studies.

Main Methods:

  • Utilized laser microdissection (LMD) technique on post-mortem human donor eyes.
  • Collected samples from the Limbal Epithelial Crypt (LEC), limbus, cornea, and conjunctiva.
  • Optimized LMD for efficient and cost-effective sample collection for both spotted oligonucleotide and Gene 1.0 ST arrays.

Main Results:

  • LMD successfully collected ocular surface epithelial tissues from various regions of the eye.
  • Achieved RNA concentrations suitable for microarray analysis, with sample areas ranging from 86,253 μm² to 1,300,000 μm².
  • RNA Integrity Numbers (RIN) for Gene 1.0 ST array samples ranged from 3.3 to 7.9, with samples below RIN 2 discarded.

Conclusions:

  • The optimized protocol significantly improved RNA yield from in situ ocular surface epithelial regions.
  • This method enables effective microarray studies using both spotted oligonucleotide and Affymetrix platforms.
  • The technique provides a reliable approach for transcriptional profiling of the ocular surface.