A genetic linkage map of papaya based on randomly amplified polymorphic DNA markers
S N Sondur1, R M Manshardt, J I Stiles
1Department of Horticulture, College of Tropical Agriculture and Human Resources, University of Hawaii, 96822, Honolulu, Hawaii, USA.
Abstract:
A genetic linkage map of papaya (Carica papaya L.) was constructed using randomly amplified polymorphic DNA (RAPD) markers and a F2 population derived from a University of Hawaii UH breeding line 356 x 'Sunrise' cross. A total of 596 10-mer primers were screened, and 96 polymorphisms were detected. At LOD 4.0, 62 of these markers mapped to 11 linkage groups comprising 999.3 cM. About 80% of the markers conformed to expected Mendelian segregation ratios. We have mapped the locus that determines sex to a 14-cM region flanked by RAPD markers. The results demonstrate the usefulness of RAPD markers for developing a basic genetic linkage map in papaya.
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