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In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

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Related Experiment Video

Updated: Mar 13, 2026

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Published on: June 25, 2021

Hybridization histochemistry.

J P Coghlan, P Aldred, J Haralambidis

Analytical Biochemistry

|August 15, 1985

Summary

This summary is machine-generated.

Hybridization histochemistry offers high specificity for locating gene expression and viral replication in single cells. This technique is becoming a valuable research tool and a potential key diagnostic aid for detecting cancer-related genes and difficult-to-detect viruses.

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Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

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Last Updated: Mar 13, 2026

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

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Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

Area of Science:

Molecular Biology
Histochemistry
Genetics

Background:

Hybridization histochemistry has seen a resurgence in interest.
It offers solutions for problems not addressed by other techniques.
Advances have made it an attractive research tool since 1978.

Purpose of the Study:

To review the advancements and applications of hybridization histochemistry.
To highlight its potential as a research tool and diagnostic aid.
To showcase its ability to locate gene expression and viral replication.

Main Methods:

Utilizing 32P-labeled probes for high-resolution in situ labeling.
Employing whole-mouse sections for broad gene expression analysis.
Using synthetic DNA (oligonucleotides) to broaden accessibility.

Main Results:

Achieved resolution down to single cells in thin sections.
Demonstrated utility in studying hormonal, enzymatic, and growth factor gene expression.
Highlighted potential in developmental studies and viral infection examination.

Conclusions:

Hybridization histochemistry provides uniquely specific anatomical localization of gene expression and viral replication.
The technique is becoming more accessible with synthetic DNA probes.
It is poised to become a key diagnostic aid, especially for oncogenes and difficult-to-detect viruses.