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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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The maize two-dimensional gel protein database: towards an integrated genome analysis program.

P Touzet1, F Riccardi, C Morin

  • 1INRA/UPS/INA P-G/CNRS-URA 1492, la Ferme du Moulon, Station de Génétique Végétale, 91190, Gif-sur-Yvette, France.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|October 29, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces the first maize protein database, identifying novel proteins and their functions using microsequencing and amino acid analysis. The database aids in mapping the maize expressed genome.

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Area of Science:

  • Proteomics
  • Genomics
  • Plant Biology

Background:

  • Two-dimensional electrophoresis is a key technique for protein separation.
  • Understanding maize protein expression is crucial for genetic research.

Purpose of the Study:

  • To establish the first comprehensive maize protein database.
  • To identify novel maize proteins and their functions.
  • To support the genetic mapping of the maize expressed genome.

Main Methods:

  • Protein separation using two-dimensional electrophoresis.
  • Partial microsequencing of identified proteins.
  • Amino acid analysis for protein identification.
  • Database creation integrating protein and genetic information.

Main Results:

  • 56 coleoptile and 18 leaf proteins from two maize lines were analyzed.
  • 49% of sequenced proteins showed similarity to known database proteins.
  • 9 novel proteins with five distinct functions were identified in maize.
  • Conclusive functions for 61% of microsequenced proteins remain undetermined.
  • Amino acid analysis proposed candidates for 17 additional coleoptile proteins.

Conclusions:

  • The developed maize protein database is a valuable resource for plant science.
  • The database facilitates the identification of novel proteins and functional characterization.
  • This resource supports ongoing projects in maize genetic mapping and expressed genome analysis.