Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 6, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

6.6K

Microsatellite DNA markers for rice chromosomes.

H Akagi1, Y Yokozeki, A Inagaki

  • 1Plant Biotechnology Laboratory, Life Science Institute, Mitsui Toatsu Chemicals Inc., Togo, 1144, 297, Mobara, Japan.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|October 29, 2013
PubMed
Summary

Researchers identified 369 microsatellites in rice DNA, developing 35 new DNA markers. These markers aid in breeding new rice varieties by enabling selection of desired traits and covering 35% of the rice genome.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outpatient Treatment of Adolescents in Japan with Drugs for Attention Deficit Disorders.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

Outpatient Treatment of Adolescents with Antidepressants in Japan.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.

Pflugers Archiv : European journal of physiology·2016
Same author

t(14;16)-positive multiple myeloma shows negativity for CD56 expression and unfavorable outcome even in the era of novel drugs.

Blood cancer journal·2015
Same author

Visible-light-controlled homo- and copolymerization of styrenes by a bichromophoric Ir-Pd catalyst.

Chemical communications (Cambridge, England)·2015
Same author

Clinical study of tympanostomy tube placement for patients with intractable Ménière's disease.

The Journal of laryngology and otology·2015

Area of Science:

  • Genomics
  • Plant Science
  • Molecular Biology

Background:

  • Microsatellites are valuable genetic markers for plant breeding.
  • Developing new microsatellite markers can be time-consuming and labor-intensive.
  • Published genomic sequences offer a potential resource for marker development.

Purpose of the Study:

  • To identify and characterize microsatellites in rice genomic sequences.
  • To develop novel microsatellite DNA markers for rice genetic studies.
  • To assess the utility of these markers for genome mapping and breeding.

Main Methods:

  • Bioinformatic analysis of 11,798 rice sequences to identify microsatellites.
  • Conversion of identified microsatellites into DNA markers using flanking sequences.

More Related Videos

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.8K
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

8.6K

Related Experiment Videos

Last Updated: May 6, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

6.6K
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.8K
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

8.6K
  • PCR-based assay and genome mapping of developed polymorphic markers between japonica and indica rice varieties.

    • Main Results:

    • Identified 369 complete microsatellites, with (CGG/GCC)n being the most frequent.
    • Successfully developed 35 new microsatellite DNA markers from 45 identified microsatellites.
    • Mapped 28 polymorphic markers on the rice genome, covering approximately 35% of the chromosomes with existing markers.
    • Demonstrated hypervariability and ease of assay for PCR-based analysis.

    Conclusions:

    • Published rice sequences are a valuable resource for developing cost-effective microsatellite DNA markers.
    • The developed microsatellite markers are well-distributed across the rice genome and useful for genetic mapping.
    • These markers can significantly aid in the development of new rice breeds by facilitating marker-assisted selection, saving time, labor, and resources.