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Updated: May 6, 2026

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
Published on: March 23, 2021
Image analysis of chromatin remodelling
Benedicto de Campos Vidal1, Marina B Felisbino, Maria Luiza S Mello
1Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
Abstract:
Chromatin packaging plays a significant role in regulating gene transcription. Study of the higher-order packing states of chromatin by image analysis at the light microscope level, especially when validated by methods of molecular biology, immunochemistry, and/or immunocytochemistry, enabled the detection of changes involved in the processes associated with or preceding alterations in transcriptional activities. Here, we recommend and describe the use of relatively simple methods for staining and detecting chromatin remodelling by image analysis.
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