Deep sequencing of small chromatin-associated RNA: isolation and library preparation
Sarah Daniela Diermeier1, Thomas Schubert, Gernot Längst
1Biochemistry Centre, Universität Regensburg, Regensburg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|October 29, 2013
Abstract:
Chromatin-associated RNA (caRNA) is a newly identified class of RNA molecules stably associated with chromatin, maintaining the higher order structure of euchromatic regions in an accessible form (Schubert et al., Mol Cell, doi:10.1016/j.molcel.2012.08.021, 2012). This section provides a detailed protocol describing the isolation of small caRNA from Drosophila cells and preparation of libraries suited for stranded small (20-200 bp) RNA deep sequencing on the Illumina platform.


