Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Opioid Receptors: Overview01:22

Opioid Receptors: Overview

7.4K
Opioid receptors, including the mu (μ, MOR), delta (δ, DOR), and kappa (κ, KOR) types, belong to the rhodopsin family of G protein-coupled receptors. These receptors are located throughout the central and peripheral nervous systems and in non-neuronal tissues such as macrophages and astrocytes. Opioid receptor ligands can be categorized into agonists or antagonists. Highly selective agonists include [d-Ala2, MePhe4, Gly(ol)5]-enkephalin or DAMGO for MOR, [D-Pen2,...
7.4K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

14.2K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
14.2K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
Drug-Receptor Interaction: Agonist01:25

Drug-Receptor Interaction: Agonist

4.4K
Agonists are drugs that interact with specific receptors in the body to produce a biological response. When an agonist binds to a receptor, it activates or enhances the receptor's function, leading to physiological effects. The interaction between agonist drugs and receptors is crucial for their therapeutic action in various medical treatments.
Agonists can bind to receptors in different ways. Some agonists bind directly to the receptor's active site, mimicking the endogenous...
4.4K
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

6.7K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
6.7K
Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship01:22

Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship

2.3K
Cholinergic agonists or cholinomimetics mimic the action of acetylcholine to stimulate the parasympathetic nervous system. They are categorized into direct-acting and indirect-acting agents. The direct-acting cholinergic drugs induce the parasympathetic response by directly binding to the muscarinic or nicotine receptors. In comparison, the indirect-acting cholinergic drugs prevent acetylcholine hydrolysis, indirectly contributing to the extended parasympathetic response.
The direct-acting...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacological modulation of TRPV2 enhances migration and induces Immunoglobulin E (IgE)-independent degranulation of mast cells.

Cell calcium·2026
Same author

Open-label placebos reduce hair cortisol concentrations and psychological distress - a randomized controlled trial.

Scientific reports·2026
Same author

O-linked glycan-dependent gating of TPC2 controls lysosomal excitability and organelle remodeling.

Nature communications·2026
Same author

[Exposure inquiries related to preparations containing plant-based ingredients in Germany: analysis of inquiries (2013-2022) at the Joint Poison Information Center in Erfurt].

Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·2026
Same author

Defining AV2-1 as a novel pharmacological probe to target human and rodent TRPV2.

British journal of pharmacology·2026
Same author

Impact of channel properties, imaging strategies and diffusional constraints on the detectability of Ca<sup>2+</sup> microdomains.

Cell calcium·2026

Related Experiment Video

Updated: May 6, 2026

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

10.3K

Natural compounds with P2X7 receptor-modulating properties.

Wolfgang Fischer1, Nicole Urban, Kerstin Immig

  • 1Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Haertelstr. 16-18, 04107, Leipzig, Germany, fisw@medizin.uni-leipzig.de.

Purinergic Signalling
|October 29, 2013
PubMed
Summary
This summary is machine-generated.

Researchers identified natural compounds that modulate the adenosine 5'-triphosphate (ATP)-gated P2X7 receptor. Teniposide blocked P2X7 activity, while agelasine and garcinolic acid enhanced it, suggesting potential therapeutic applications.

More Related Videos

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

14.0K
Author Spotlight: Developing Parmodulins to Target Protease-Activated Receptors for Inflammation Control
07:13

Author Spotlight: Developing Parmodulins to Target Protease-Activated Receptors for Inflammation Control

Published on: May 24, 2024

1.1K

Related Experiment Videos

Last Updated: May 6, 2026

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

10.3K
Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

14.0K
Author Spotlight: Developing Parmodulins to Target Protease-Activated Receptors for Inflammation Control
07:13

Author Spotlight: Developing Parmodulins to Target Protease-Activated Receptors for Inflammation Control

Published on: May 24, 2024

1.1K

Area of Science:

  • Pharmacology
  • Cell Biology
  • Neuroscience

Background:

  • The P2X7 receptor, activated by extracellular adenosine 5 -triphosphate (ATP), is a non-selective cation channel involved in immune responses, inflammation, cancer, and neurodegeneration.
  • Modulation of P2X7 receptor activity is crucial for understanding and treating various pathophysiological processes.

Purpose of the Study:

  • To identify and characterize natural compounds and their analogues that modulate the human P2X7 receptor.
  • To investigate the effects of these compounds on ATP-induced calcium ion (Ca2+) influx and dye uptake in cells expressing P2X7.

Main Methods:

  • Utilized a HEK293 cell line stably expressing human P2X7 receptors.
  • Employed fluorometric imaging plate reader technology to measure ATP-induced Ca2+ responses.
  • Assessed compound effects on ATP-stimulated Ca2+ influx and Yo-Pro-1 dye uptake in P2X7-expressing cells, including melanoma and microglial cells.

Main Results:

  • Teniposide demonstrated potent blockade of human P2X7 receptor activity at sub-micromolar concentrations.
  • Agelasine (AGL) and garcinolic acid (GA) facilitated the P2X7 receptor response to ATP in HEK293, melanoma, and microglial cells.
  • Garcinolic acid enhanced Yo-Pro-1 uptake, indicating modulation of P2X7 channel pore formation, while agelasine did not.

Conclusions:

  • Selective modulators of the P2X7 receptor, such as teniposide, agelasine, and garcinolic acid, were identified.
  • These compounds exhibit distinct effects (blockade or sensitization) on P2X7 receptor function.
  • P2X7 receptor modulators hold promise for therapeutic development in neuroprotection and cancer treatment.