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A human prostatic growth factor (hPGF): partial purification and characterization.

N Nishi, Y Matuo, Y Muguruma

    Biochemical and Biophysical Research Communications
    |November 15, 1985
    PubMed
    Summary
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    Researchers purified a novel growth factor from human benign hypertrophic prostates that stimulates DNA synthesis in BALB/3T3 cells. This prostate growth factor shows potential for further study in cell proliferation research.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • Benign prostatic hyperplasia (BPH) involves abnormal prostate cell growth.
    • Growth factors play a critical role in regulating cell proliferation.
    • Identifying specific growth factors in BPH can offer insights into disease mechanisms.

    Purpose of the Study:

    • To isolate and characterize a growth factor from human benign hypertrophic prostates.
    • To determine the growth factor's ability to stimulate DNA synthesis in BALB/3T3 cells.
    • To investigate the optimal conditions for the growth factor's activity.

    Main Methods:

    • Purification of the growth factor using heparin-Sepharose chromatography.
    • Estimation of molecular weight and isoelectric point.

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  • Assay of DNA synthesis stimulation in BALB/3T3 cells.

    • Main Results:

    • A growth factor was purified ~1,000-fold from human benign hypertrophic prostates.
    • The purified factor stimulated DNA synthesis in BALB/3T3 cells at 10 ng/ml.
    • Growth factor activity was dependent on serum concentration, increasing 5-fold with higher serum levels.

    Conclusions:

    • A potent DNA synthesis-stimulating growth factor was identified in benign hypertrophic prostate cytosol.
    • The factor's properties (MW 11-13 kDa, pI 10.5) were characterized.
    • This growth factor may play a role in prostate tissue proliferation.