Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Local base dynamics and local structural features in RNA and DNA duplexes.

S C Kao, A M Bobst

    Biochemistry
    |September 24, 1985
    PubMed
    Summary

    Spin-labeling reveals nucleotide base motion in RNA and DNA. Base pairing significantly reduces base mobility, with slower probe motion observed in RNA duplexes compared to DNA.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Measurement of the triple-differential dijet cross section in proton-proton collisions at <math> </math> and constraints on parton distribution functions.

    The European physical journal. C, Particles and fields·2020
    Same author

    Measurements of the associated production of a Z boson and b jets in pp collisions at <math> </math>.

    The European physical journal. C, Particles and fields·2020
    Same author

    Search for dark matter produced in association with heavy-flavor quark pairs in proton-proton collisions at <math> </math>.

    The European physical journal. C, Particles and fields·2020
    Same author

    Study of dijet events with a large rapidity gap between the two leading jets in pp collisions at <math> </math> <math></math>.

    The European physical journal. C, Particles and fields·2019
    Same author

    Measurement of associated Z + charm production in proton-proton collisions at <math> </math> <math></math>.

    The European physical journal. C, Particles and fields·2019
    Same author

    Suppression of Excited ϒ States Relative to the Ground State in Pb-Pb Collisions at sqrt[s]_{NN}=5.02  TeV.

    Physical review letters·2018

    Area of Science:

    • Biophysics
    • Molecular Biology
    • Structural Biology

    Background:

    • Understanding nucleic acid dynamics is crucial for biological function.
    • Site-specific spin-labeling provides insights into local molecular motion.

    Purpose of the Study:

    • To analyze local base motion and structural dynamics in various nucleic acid systems.
    • To quantify the effect of base pairing on nucleotide mobility.

    Main Methods:

    • Application of a simple motional model to site-specifically spin-labeled polyribonucleotides and polydeoxyribonucleotides.
    • Analysis of 22 different nucleic acid systems.

    Main Results:

    • Nucleotide base motion in single-stranded RNA, DNA, or non-base-paired duplex bases occurs on the order of 1 nanosecond.
    • Base pairing decreases nucleotide base mobility by approximately a factor of 4.
    • Tether motion of the spin-label probe is slower in RNA duplexes than in DNA duplexes.

    Conclusions:

    • Base pairing significantly restricts local base motion in nucleic acids.
    • Differences in probe motion between RNA and DNA duplexes suggest distinct dynamic properties.

    Related Experiment Videos