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Characterization of individual nano-objects with nanoprojectile-SIMS.

C-K Liang1, S V Verkhoturov, Y Bisrat

  • 1Department of Chemistry, Texas A&M University, College Station, TX, 77843-3255, USA.

Surface and Interface Analysis : SIA
|October 29, 2013
PubMed
Summary

Secondary ion mass spectrometry (SIMS) enables individual nano-object characterization by analyzing them one-by-one. A Nano-Assisted Laser Desorption/Ionization (NALDI™) plate effectively isolates these nano-objects for precise compositional analysis.

Keywords:
NALDI™ToF-SIMSevent-by-event SIMS analysisgold clustersnano-objects

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biophysics

Background:

  • Individual nano-object analysis requires methods capable of detecting compositional variations.
  • Existing techniques often struggle with isolating nano-objects for single-particle characterization.
  • The development of specialized substrates is crucial for advancing nano-object analysis.

Purpose of the Study:

  • To demonstrate the capability of event-by-event Secondary Ion Mass Spectrometry (SIMS) for individual nano-object characterization.
  • To validate the use of a Nano-Assisted Laser Desorption/Ionization (NALDI™) plate as a suitable substrate for isolating nano-objects.
  • To analyze the composition of individual bacteriophages (Qβ and M13) using this methodology.

Main Methods:

  • Utilized event-by-event SIMS for high-resolution analysis of individual nano-objects.
  • Employed a NALDI™ plate with a nanostructured surface for nano-object deposition and isolation.
  • Performed Scanning Electron Microscopy (SEM) to verify the integrity of nano-objects on the substrate.

Main Results:

  • Successfully isolated individual nano-objects, specifically Qβ and M13 bacteriophages, on the NALDI™ plate.
  • Confirmed the preservation of phage integrity using SEM imaging.
  • Obtained mass spectrometric data clearly identifying secondary ions from the phages, distinct from the substrate.

Conclusions:

  • Event-by-event SIMS combined with NALDI™ plates is a powerful approach for characterizing individual nano-objects.
  • The NALDI™ substrate effectively facilitates the isolation of nano-objects for single-particle analysis.
  • This method allows for the detailed compositional analysis of nanometric biological entities like bacteriophages.