Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

9.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
9.6K
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

1.5K
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
1.5K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.6K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.6K
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

2.0K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
2.0K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

1.5K
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
1.5K
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

6.2K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Rise of Trapped Ion Mobility Spectrometry: Principles, Applications, and Recent Developments.

Mass spectrometry reviews·2026
Same author

Legends of Ion Mobility Spectrometry - Martin F. Jarrold.

Journal of the American Society for Mass Spectrometry·2026
Same author

Legends of Ion Mobility Spectrometry - Glenn E. Spangler.

Journal of the American Society for Mass Spectrometry·2026
Same author

Structural Landscape of Syanodin I Lasso Peptide.

Biochemistry·2026
Same author

Legends of Ion Mobility Spectrometry - Gary A. Eiceman.

Journal of the American Society for Mass Spectrometry·2026
Same author

Interactions between long-acting antiretrovirals and opioids: a call for clinical awareness.

Clinical microbiology reviews·2026
Same journal

Modular and reconfigurable gas chromatography / differential mobility spectrometry (GC/DMS) package for detection of volatile organic compounds (VOCs).

International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry·2019
Same journal

Utilizing Ion Mobility to Identify Isobaric Post-Translational Modifications: Resolving Acrolein and Propionyl Lysine Adducts by TIMS Mass Spectrometry.

International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry·2018
Same journal

Towards the Analysis of High Molecular Weight Proteins and Protein complexes using TIMS-MS.

International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry·2016
Same journal

Supervised Semi-Automated Data Analysis Software for Gas Chromatography / Differential Mobility Spectrometry (GC/DMS) Metabolomics Applications.

International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry·2016
Same journal

Gated Trapped Ion Mobility Spectrometry Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.

International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry·2016
Same journal

Isomer Separation of Polybrominated Diphenyl Ether Metabolites using nanoESI-TIMS-MS.

International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry·2016
See all related articles

Related Experiment Video

Updated: May 6, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

3.8K

Gas-phase separation using a trapped ion mobility spectrometer.

Francisco Fernandez-Lima1, Desmond A Kaplan, J Suetering

  • 1Department of Chemistry, Texas A&M University, MS 3144, College Station, TX 77843-3144, USA.

International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry
|October 29, 2013
PubMed
Summary
This summary is machine-generated.

Trapped Ion Mobility Spectrometry (TIMS) offers fast gas-phase ion separation by size-to-charge ratio. This versatile technique provides tunable resolution and seamless integration with mass spectrometry for enhanced analysis.

Keywords:
Ion trappingMobility separationRadial focusing

More Related Videos

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.6K
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

27.2K

Related Experiment Videos

Last Updated: May 6, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

3.8K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.6K
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

27.2K

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Ion mobility spectrometry (IMS) separates ions in the gas phase.
  • Traditional drift tube IMS has limitations in resolution and integration.

Purpose of the Study:

  • To describe the principles, versatility, and applicability of Trapped Ion Mobility Spectrometry (TIMS).
  • To demonstrate TIMS for fast, gas-phase separation of molecular ions and isobar pairs.

Main Methods:

  • Utilized a Trapped Ion Mobility Spectrometer (TIMS) for ion separation.
  • Investigated the dependence of mobility resolution on bath gas velocity and scan speed.
  • Demonstrated tunable resolution (R>50) based on analytical needs.

Main Results:

  • Achieved fast, gas-phase separation of molecular ions based on size-to-charge ratio.
  • Successfully separated isobar pairs using TIMS.
  • Showcased the tunability of TIMS resolution for different analytical challenges.

Conclusions:

  • TIMS provides a versatile and efficient platform for gas-phase ion separation.
  • TIMS offers tunable resolution and easy integration with mass spectrometers.
  • TIMS enhances analytical capabilities by providing a powerful separation tool prior to mass analysis.