Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

919
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
919

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-omics Signature Predicts Anti-EGFR Therapy Benefit in Colorectal Cancer Liver Metastases: A Multi-center Cohort Study.

Current cancer drug targets·2026
Same author

Minimally invasive extraperitoneal posterior neurectomy for anterior cutaneous nerve entrapment syndrome (ACNES): Primary description of the MEPONE technique and clinical outcomes.

Hernia : the journal of hernias and abdominal wall surgery·2026
Same author

Adherence to 24-hour movement guidelines and mortality risks in US cancer survivors: a national cohort study.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

Efficacy of Renin-Angiotensin System Inhibitors in Long-Term Oncologic Outcomes of Patients With Stage II/III Colon Cancer and Hypertension: A Target Trial Emulation.

JCO oncology practice·2026
Same author

Prognostic value of quantitative multiparametric magnetic resonance imaging parameters and histopathologic features for disease-free survival in locally advanced non-mucinous rectal adenocarcinoma.

Abdominal radiology (New York)·2026
Same author

Additive and Multiplicative Effects of Socially Stigmatized Identities Using Linear Regression to Model Effects on Self-Reported Overall Health as Reported in the All of Us Research Program: Quantitative Analysis.

JMIR formative research·2026
Same journal

A high-density 3D localization algorithm for stochastic optical reconstruction microscopy.

Optical nanoscopy·2014
Same journal

Active Microscope Stabilization in Three Dimensions Using Image Correlation.

Optical nanoscopy·2014
Same journal

Resolving the spatial relationship between intracellular components by dual color super resolution optical fluctuations imaging (SOFI).

Optical nanoscopy·2013
Same journal

Light-sheet Bayesian microscopy enables deep-cell super-resolution imaging of heterochromatin in live human embryonic stem cells.

Optical nanoscopy·2013
See all related articles

Related Experiment Video

Updated: May 6, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

790

SOFI-based 3D superresolution sectioning with a widefield microscope.

Thomas Dertinger1, Jianmin Xu, Omeed Foroutan Naini

  • 1Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA ; SOFast GmbH, Dresdener Str 14, 10999 Berlin, Germany.

Optical Nanoscopy
|October 29, 2013
PubMed
Summary
This summary is machine-generated.

Superresolution optical fluctuation imaging (SOFI) enables 3D superresolution imaging and sectioning on conventional microscopes. This technique transforms standard widefield microscopes into powerful superresolution tools for biological research.

More Related Videos

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

21.7K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.0K

Related Experiment Videos

Last Updated: May 6, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

790
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

21.7K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.0K

Area of Science:

  • Optical microscopy
  • Biophysics
  • Cell biology

Background:

  • Superresolution microscopy has advanced biological imaging.
  • Achieving 3D superresolution remains challenging with current methods.
  • Conventional widefield microscopes lack superresolution capabilities.

Purpose of the Study:

  • To demonstrate a facile method for 3D superresolution imaging.
  • To adapt superresolution optical fluctuation imaging (SOFI) for 3D imaging.
  • To image and section the cytoskeletal network of fixed cells.

Main Methods:

  • Utilized superresolution optical fluctuation imaging (SOFI).
  • Performed SOFI on a conventional lamp-based widefield microscope.
  • Applied the technique to fixed cells for cytoskeletal network imaging.

Main Results:

  • Achieved facile and straightforward 3D superresolution imaging.
  • Demonstrated effective sectioning of cellular structures.
  • Successfully imaged the cytoskeletal network with high resolution.

Conclusions:

  • SOFI possesses inherent sectioning capabilities.
  • SOFI transforms conventional widefield microscopes into superresolution instruments.
  • This method offers a practical approach to 3D superresolution imaging.