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Updated: May 6, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[Identification of pathogenic microorganism by sequencing 16S rRNA gene]
Feizhou Zhu1, Liyu Chen, Hanchun Chen
1Department of Biochemistry, School of Life Sciences, School of Basic Medicine, Central South University, Changsha 410013, China.
Objective:
To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future.
Methods:
DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleotide database. The bacteria were identified according to the homology of their 16S rRNA genes.
Results:
Twelve bacteria were classified to species, the other 2 bacteria were classified to genus.
Conclusion:
16S rRNA gene sequence analysis is useful in identifying pathogenic bacteria.
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