Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.2K
Computed Tomography01:10

Computed Tomography

7.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.6K
Group Polarization01:01

Group Polarization

31.3K
Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.
31.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In vivo</i> characterization of blue-light-induced peripheral arterial photorelaxation using functional OCT.

Biomedical optics express·2026
Same author

Dual-color augmented reality waveguide display for color vision assistance using color tracking.

iScience·2026
Same author

Optical coherence tomography angiography reveals structural and hemodynamic remodeling of cerebral penetrating vessels owing to aging.

Biomedical optics express·2026
Same author

Frequency-domain photoacoustic microscopy with resonant transducer and interferometric modulation for high-reliability sO<sub>2</sub> imaging.

Photoacoustics·2026
Same author

Physics-consistent denoising of full-field photoacoustic displacement maps using an adaptive residual attention network.

Photoacoustics·2026
Same author

Off-axis varifocal augmented reality near-eye display with holographic astigmatism compensation.

Optics express·2026

Related Experiment Video

Updated: May 6, 2026

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

10.9K

Single-camera polarization-sensitive full-field optical coherence tomography with polarization switch.

Kwan Seob Park1, Woo June Choi, Tae Joong Eom

  • 1Gwangju Institute of Science and Technology, School of Information and Communications, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.

Journal of Biomedical Optics
|October 30, 2013
PubMed
Summary

We developed a new polarization-sensitive full-field optical coherence tomography (PS-FF-OCT) system. This advanced imaging technique simultaneously captures high-resolution OCT and linear retardance images of biological tissues.

More Related Videos

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

4.0K
Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.0K

Related Experiment Videos

Last Updated: May 6, 2026

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

10.9K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

4.0K
Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.0K

Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Biophysics

Background:

  • Optical Coherence Tomography (OCT) provides high-resolution cross-sectional imaging.
  • Polarization-sensitive OCT (PS-OCT) enhances contrast and provides tissue microstructural information.
  • Full-field OCT (FF-OCT) offers parallel acquisition of large areas, improving imaging speed.

Purpose of the Study:

  • To develop and demonstrate a single-channel detection-based polarization-sensitive full-field optical coherence tomography (PS-FF-OCT) system.
  • To achieve simultaneous acquisition of high-resolution OCT and linear retardance images.
  • To validate the system's performance using a biological sample.

Main Methods:

  • Utilized a sub-10-fs laser as a broadband light source for OCT.
  • Employed a bi-stable polarization switching device with a ferroelectric liquid crystal cell and analyzer.
  • Sequentially recorded time-switched perpendicular polarization interference signals using a single CCD camera.

Main Results:

  • Successfully extracted en-face functional images of a biological sample.
  • Demonstrated imaging feasibility with ex vivo rat tail tendon.
  • Achieved axial resolution of 2 μm and transverse resolution of 1.3 μm.

Conclusions:

  • The developed single-channel PS-FF-OCT system enables simultaneous high-resolution OCT and linear retardance imaging.
  • The system offers potential for advanced biomedical imaging applications requiring detailed microstructural and polarization information.
  • The achieved resolutions confirm the system's capability for fine biological tissue analysis.