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Updated: May 6, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Synthesis of nitric oxide probes with fluorescence lifetime sensitivity
Natalia G Zhegalova1, Garrett Gonzales, Mikhail Y Berezin
1Department of Radiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110, USA. berezinm@mir.wustl.edu.
Abstract:
We present the rationale, synthesis and evaluation of the first activatable fluorescent probe that utilizes fluorescence lifetime change for detection of nitric oxide. The new probe DAP-LT1 features a near-infrared polymethine skeleton with a diaminobenzene functionality incorporated into the meso-position. The probe is partially quenched, and upon reaction with nitric oxide shows an increase in the fluorescence lifetime from 1.08 ns to 1.24 ns.
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