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Screening apples for OPD20/600 using sequence-specific primers.

H Yang1, S S Korban

  • 1Department of Horticulture, University of Illinois, 310 Madigan Bldg., 1201 W. Gregory Drive, 61801, Urbana, IL, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|October 30, 2013
PubMed
Summary
This summary is machine-generated.

Researchers identified a DNA marker linked to apple scab resistance in Malus floribunda 821. This marker, OPD20/600, aids in breeding disease-resistant apple varieties by detecting the Vf gene.

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Area of Science:

  • Plant Pathology
  • Molecular Genetics
  • Horticultural Science

Background:

  • Apple scab, caused by Venturia inaequalis, is a major global threat to apple production.
  • Resistance to apple scab can be conferred by the Vf gene, originating from Malus floribunda 821.

Purpose of the Study:

  • To identify and characterize DNA markers linked to the Vf gene for apple scab resistance.
  • To evaluate the utility of sequence-specific primers for marker-assisted selection in apple breeding.

Main Methods:

  • Random Amplified Polymorphic DNA (RAPD) analysis was used to identify an initial marker (OPD20/600) linked to the Vf gene.
  • The identified marker was cloned, sequenced, and used to design sequence-specific primers.
  • These primers were employed to screen various apple genotypes for polymorphisms related to the Vf gene.

Main Results:

  • A RAPD marker, OPD20/600, was found to be linked to the Vf gene conferring resistance to apple scab.
  • Sequence-specific primers derived from OPD20/600 successfully identified polymorphisms based on band presence or absence.
  • This method allowed for the screening of diverse apple cultivars and selections for scab resistance.

Conclusions:

  • Sequence-specific primers offer an efficient and reliable method for developing genetic markers linked to important traits like disease resistance.
  • The identified marker and developed primers can facilitate marker-assisted selection for breeding scab-resistant apple trees.
  • This approach enhances the development of improved apple cultivars with durable resistance to Venturia inaequalis.