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Updated: May 12, 2026

In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity
Vera Bilan1, Danilo Allegra, Florian Kuchenbauer
1Internal Medicine III, University of Ulm, Ulm, Germany.
Abstract:
Luciferase reporter assays are widely used to study promoter activity, transcription factors, intracellular signaling, protein interactions (Jia et al., PloS One 6:e26414), miRNA processing (Allegra and Mertens, Biochem Biophys Res Commun 406:501-505), and target recognition (Jin et al., Methods Mol Biol 936:117-127). Here we describe the use of a dual-luciferase reporter system to evaluate the enzymatic activity of a key enzyme involved in RNA maturation-DROSHA. This dual system is a simple and fast method for the quantification of the DROSHA processing activity in live cells.
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