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Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture
M Hannah Degeling1, M Sarah S Bovenberg, Marie Tannous
1Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 30, 2013
Abstract:
Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.
