Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Testing of granulocyte function after gravity leukapheresis].

E U Steinhauer, A Steinhauer, D Jorke

    Folia Haematologica (Leipzig, Germany : 1928)
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Efficacy and safety of ibandronate in the treatment of hypercalcemia of malignancy: a randomized multicentric comparison to pamidronate.

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2003
    Same author

    Serum parathyroid hormone-related protein levels and response to bisphosphonate treatment in hypercalcemia of malignancy.

    The Journal of clinical endocrinology and metabolism·1999
    Same author

    [Diploma of continuing education from the Society of Physician of Thüringia].

    Zeitschrift fur arztliche Fortbildung und Qualitatssicherung·1998
    Same author

    Dose-response study of ibandronate in the treatment of cancer-associated hypercalcaemia.

    British journal of cancer·1997
    Same author

    [Ethical decisions in medical treatment].

    Medizinische Klinik (Munich, Germany : 1983)·1996
    Same author

    Randomized phase II trial comparing different doses of the bisphosphonate ibandronate in the treatment of hypercalcemia of malignancy.

    Journal of clinical oncology : official journal of the American Society of Clinical Oncology·1996

    Gravitational leukopheresis effectively produces granulocyte concentrates using hydroxyethyl starch (HAS) as a sedimentation accelerator. While cell viability remains high initially, functional capacities decline significantly after four hours.

    Area of Science:

    • Hematology
    • Biotechnology

    Context:

    • Granulocyte concentrates are crucial for treating certain hematological disorders and infections.
    • Production methods aim to maximize cell yield and viability while preserving function.

    Purpose:

    • To evaluate the efficacy of gravitational leukopheresis for granulocyte concentrate production.
    • To assess the impact of hydroxyethyl starch (HAS) as a sedimentation accelerator.
    • To compare the quality and function of granulocytes produced using different HAS preparations.

    Summary:

    • Gravitational leukopheresis with hydroxyethyl starch (HAS) achieved a 70% recovery rate of granulocyte concentrates with 98% cell viability.
    • Initial vitality, phagocytosis, and migration were minimally affected within 4 hours, but decreased significantly thereafter.

    Related Experiment Videos

  • No substantial differences were observed between the industrial GDR preparation and the Plasmasteril control.

    • Impact:

    • This method offers a viable approach for producing granulocyte concentrates in laboratory settings.
    • Understanding the functional decline over time is critical for optimizing transfusion timing and therapeutic outcomes.
    • The findings support the use of HAS in leukapheresis procedures, with comparable results from different sources.