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Updated: May 6, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
31.9K
Directed genome engineering for genome optimization.
Kathleen D'Halluin1, Rene Ruiter
1Bayer CropScience N.V., Gent, Belgium. Kathleen.dhalluin@bayer.com.
The International Journal of Developmental Biology
|October 30, 2013
Summary
Site-specific nucleases enable targeted DNA double-strand break induction for plant genome optimization. This technology accelerates crop improvement and functional genomics research by precisely modifying plant traits.
Area of Science:
- Plant biotechnology
- Genomics
- Molecular biology
Background:
- Site-specific nucleases are crucial for targeted genome modification in plants.
- Genomics and systems biology provide vast data for trait development.
Purpose of the Study:
- To review directed genome engineering approaches, focusing on double-strand break (DSB)-mediated engineering.
- To highlight applications in crop improvement and functional genomics.
Main Methods:
- Development of tailor-made nucleases for precise DNA double-strand break induction.
- Application of directed genome engineering technologies.
Main Results:
- Demonstrated utility in engineering disease resistance, herbicide tolerance, and altered metabolite profiles.
- Enabled molecular trait stacking for linked transgene transmission.
Conclusions:
- Directed genome engineering, particularly DSB-mediated, is a powerful tool for plant genome optimization.
- Accelerates the creation of improved crop varieties and the dissection of gene functions.
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