ADP-induced refractoriness of platelets preserved in different temperature conditions

Storage of platelets is known to cause significant cell alterations dependent on temperature. The ability of developing the refractory state in platelets exposed to different temperature conditions of preservation was investigated. PC storage at 4 degrees C was shown to produce a decrease of platelet ability to transform to the refractory state under action of ADP and a storage at 22 degrees C resulted in an increase of this ability. The inverse correlation between refractoriness and aggregation was found in platelets preserved at 22 degrees C and in combined temperature conditions. The effects observed were supposed to be related to changes in the platelet adenylate cyclase system.