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Updated: May 6, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in
Quentin Ledoux1, Pascal Veys2, Pierre Van Cutsem3
1Département Valorisation des Productions Agricoles; Centre Wallon de Recherches Agronomiques; Gembloux, Belgium; Laboratoire de Chimie Organique et Médicinale; Université catholique de Louvain; Louvain-la-Neuve, Belgium.
Abstract:
Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

