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Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Adrenomedullin expression in the developing human fetal lung.

Carlos G Ramos¹, Xi Sun, Eric B Johnson

¹From the *Division of Neonatology, Department of Pediatrics, School of Medicine, University of New Mexico; †Biostatistics Core, School of Medicine, University of New Mexico; ‡Department of Cell Biology and Physiology, School of Medicine, University of New Mexico, Albuquerque, NM.

Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research

|October 31, 2013

Summary

Adrenomedullin (AM) is present in developing human lungs. Its expression and that of its AM1 receptor components increase with gestational age, suggesting a key role in lung development.

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Area of Science:

Pulmonary Medicine
Developmental Biology
Endocrinology

Background:

Adrenomedullin (AM) is a bioactive peptide with known roles in cardiovascular and respiratory health.
Its specific function in human fetal lung development, including vascular and alveolar growth, remains largely uncharacterized.

Purpose of the Study:

To investigate the expression patterns of Adrenomedullin (AM) and its receptor components, calcitonin-receptor-like receptor (CRLR), receptor activity-modifying protein (RAMP)2, and RAMP3, in the developing human fetal lung.
To determine if AM expression changes with advancing gestational age (10–24 weeks).

Main Methods:

Quantitative analysis of messenger RNA (mRNA) for AM, CRLR, RAMP2, and RAMP3 using real-time reverse-transcription polymerase chain reaction (RT-PCR).
Assessment of Adrenomedullin protein levels via Western blot analysis.
Immunohistochemical localization of AM and its receptor components within fetal lung tissue sections.

Main Results:

Transcripts for AM, CRLR, RAMP2, and RAMP3 were detected in midgestation human fetal lungs.
Gene expression of AM, CRLR, and RAMP2 significantly increased with advancing gestational age.
Conversely, RAMP3 gene expression decreased as gestation progressed, and Adrenomedullin protein levels also showed an increase.

Conclusions:

Adrenomedullin is expressed in the midgestation human fetal lung, with increasing gene and protein expression correlating with gestational age.
The observed upregulation of AM, CRLR, and RAMP2 suggests a crucial role for the AM1 receptor pathway in human lung development.
The differential expression of RAMP3 indicates distinct roles for AM2 receptors, warranting further investigation into their specific functions in fetal lung development.