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Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns
K Wolff1, E Zietkiewicz, H Hofstra
1Institute of Evolutionary and Ecological Sciences, University of Leiden, PO Box 9516, 2300, RA Leiden, the Netherlands.
Abstract:
Several techniques of DNA analysis were applied to identify chrysanthemum cultivars. Unrelated cultivars could be distinguished by using RAPDs (random amplified polymorphic DNAs), inter-SSR (simple sequence repeat) PCR (polymerase chain reaction), hybridization-based DNA fingerprinting, as well as RFLPs (restriction fragment length polymorphisms). Cultivars with different flower colours and belonging to one family, i.e. vegetatively derived from 1 cultivar, appeared to have the same DNA fragment patterns, whichever technique was applied. The absence of polymorphisms between different accessions of the same cultivar indicated a high stability of the observed patterns.
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