Genetic localisation of transformation competence in diploid potato
A El-Kharbotly1, E Jacobsen, W J Stiekema
1Department of Plant Breeding, The Graduate School of Experimental Plant Sciences, Agricultural University Wageningen, P. O. Box 386, NL-6700, AJ Wageningen, The Netherlands.
Abstract:
In the course of improving diploid potato genotypes for transformation ability, selection for specific components affecting regeneration and transformation was carried out. From a segregating population between two good regenerating clones a selection was made to yield an optimal well-transforming and fertile genotype J92-6400-A16. This plant yielded predominantly diploid transformants and was heterozygous for the gene R1, conferring resistance to Phytophthora infestans. The speed of, and competence for, regeneration and transformation on both sides of the stem explant were improved. A competence factor for tranformation was found to be linked with the R1 locus and a molecular marker on chromosome 5. The male fertility of transformants was frequently decreased to a great extent, whereas female fertility was not so markedly affected.
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